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KMID : 0043320010240040316
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Archives of Pharmacal Research
2001 Volume.24 No. 4 p.316 ~ p.322
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Cloning, Sequence Analysis, and Charafterization of the astA Gene Encoding an Arylsulfate Sulfotransferase from Cifraflader freundii
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Kang JW
Jeong YJ/Kwon AR/Yun HJ/Kim DH/Choi EC
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Abstract
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Arylsulfate sulfotransferase (ASST) transfers a sulfate group from a phenolic sulfate ester to a phenolic acceptor substrate. In the present studys the gene encoding ASST was c loned from a genomic library copy of Citrobacter freundii freundii, subcloned into the vector pGEM3Zf(-) and sequenced. Sequencing revealed two contiguous open reading frames (ORFI and ORF2) on the same strand and based on amino acid sequence homology, they were designated as astA and dsbA, respective1y. The amino acid sequence of astA Deduced from f.Freundii was highly si milan to that of the Safmonella typhimurium, Enterobacter amnigenus, Klebsiella, Pseudomonas putida, and Campylobacter jejuni, encoded by the astA genes. However, the ASST activity assay revealed different acceptor specificities. Using p- nitrophenyl sulfate (PNS) as a donor substrate, α-naphthol was found to be the best accepter substrate, followed by phenol, resorcinol, p-acetaminophen, tyramine and tyrosine.
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